OTE Stain
(Oolong Tea Extract)
The OTE is a non-toxic and non-radioactive stain.
The OTE is effective for fibrous connective tissues.
The solution may be stored in a fridge for 2 days for further use, but the use of freshly prepared solution is highly recommended to get better results.
332 OTE powder 10g
Refernce article
Use of oolong tea extract(OTE) for elastin and enhancement in ultrathin sections
Med Electron Microsc(2003)36:179-182
Shigeru Sato, Yoshihiro Sasaki, Akiko Adachi,
Wei Dai, Xiao-Lan Liu, Shigeki Namimatsu
Oolong tea extract as a substitute for uranyl acetate in staining of ultrathin sections
Journal of Microscopy, vol. 229,
Pt1 2008. pp.17-20
Wistar rat lung
Elastic Laminate (EL)
Electron-dense Filaments (arrows)
OTE(using buffered) + Lead Citrate
picture provider : Dr.Shigeru Sato,
Central Institute for Electron Microscopy,
Nippon Medical School
How to use
1. Staining of thin slices for TEM observation
EPreparation of the stain solution
Dissolve OTE powder either in 0.1M phosphate buffer or in 0.1M cacodylate buffer to make a solution containing approx. 0.2 wt% OTE which corresponds to a saturated concentration of OTE in these solutions at room temperature.
EProcedure for single side staining
Place the copper grid with slices on the surface of a drop of the stain solution for 15`20 minutes as shown schematically below. For the cases where enhancement of contrast is required, longer staining time, 20`30 minutes, is recommended. Then, immerse the grid fully into a drop of distilled water, leave it for 5`10 minutes to remove contamination completely before proceeding onto post-staining with uranyl acetate and lead citrate.
2.Conductive staining for SEM examination
Dissolve OTE powder in 0.1M phosphate buffer solution containing 1` 2.5% glutaraldehyde to make a solution containing approx. 0.2wt% OTE.
Samples are stained in two ways depending on their nature;
(1)Immerse the sample directly into the solution and leave it for approx. 2hrs for fixation or
(2)as (1), but after pre-immersion in 0.1M phosphate (cacodylate) buffer solution containing 2.5% glutaraldehyde for approx.10 minutes.
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